Beginning in 1929, intrastate bus service was regulated by the Florida Railroad Commission, later the Florida Public Service Commission. Information could not be located, regarding the issuance of Certificates of Convenience and Necessity for bus service. But the February 1923 issue of the publication "Bus Transportation" included a table listing the bus routes in existence in Florida.
Not listed are city bus routes, along with bus routes operating strictly within one county.
This information does not include the actual bus companies operating most of the routes. For most routes it was not possible, to establish how these companies became part of the Greyhound or other larger bus companies.
One of the earliest intercity bus routes in Florida was between Jacksonville and Miami, which evolved to Florida Greyhound Lines.
Avon Park-Haines City Carbur-Madison Daytona-Jacksonville Daytona-West Palm Beach De Funiak Springs-Florala AL Fort Myers-Moore Haven Fort Pierce-Lake Okeechobee Freeport-De Funiak Springs Green Grove Springs-Jacksonville Greenville-Perry Jacksonville-Fernandina Jacksonville-Lake City Jacksonville-Miami Jacksonville-St. Augustine Lakeland-Arcadia Lake Wales-Lakeland Madison-Sparks GA Madison-Tifton GA Marianna-Dothan AL Marianna-Panama City Mayo-Live Oak Miami-West Palm Beach Milton-Florala AL Orlando-Daytona Orlando-Leesburg Palatka-Ocala Palatka-Gainesville Plant City-Fort Meade St. Augustine-Palatka St. Petersburg-Tampa Sebring-Wauchula Tampa-Sarasota Tampa-Orlando Tampa-Lakeland Tampa-Tarpon Springs Weewahitchka-Marianna